Herb Compatible Action of Fuzheng Huayu 319 Decoction on Albumin Production in Hepatocytes
LIU Cheng-hai
WANG Xiao-ling
TAN Yin-Zi
WANG Zeng-nan
LIU Ping
Abstract：Aims:To investigate the mechanism of fuzheng Huayu 319 decoction and the compatibility of medicine in the decoction on albumin(Alb) production in hepatocytes. Methods:the hepatocytes were isolated through in situ liver perfusion with collagen from rats,and primarily cultured. Fuzheng Huayu 319 decoction(319 decoction) was divided into 5 subgroups:Fuzheng,Huayu,salvia miltiorrhizae,cordyceps,and salvia miltiorrhizae puls cordyceps. The drug sera were collected from rats that were feed on 319 decoction and its subgroups respectively, and incubated with cultured cells. The albumin(Alb) contents in culture medium were measured by ELISA, and were normalized by the total protein in the cell layer. The pre-Alb mRNA expression was analyzed by RT-PCR. Results:All drug sera had no obvious influence on the cell morphology,and promoted Alb secretion and pre-Alb mRNA expression in cultured hepatocytes in different degrees. Among them,319 decoction effect was the strongest,and the effects of Fuzheng and Huayu subgroup were better than respectively. Conclusion: the promotion of pre-Alb gene expression was the main mechanism of 319 decoction on Alb production. On this effect of 319 decoction,the combination of Fuzheng and Huayu drugs had comprehensive advantages over the single treatment group or single herb.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 36-39 )