Study of the Molecular Basis of the Theory that “The Kidney Produces the Marrow” by Measuring the Transcription Level of Granulocyte-Macrophagocyte Colony-Stimulating Factor Gene of Aged Mice Medicated with Bu Shen Sheng Xue Yao
CHEN Zhi-song
WU Zhi-kui
CAI Hui-guo
XU Jing
CHEN Yu-ying
LU Xin-xia
Abstract：Objective: In order to explore the molecular basis of the theory that “The kidney Produces the Marrow” and clarify the essential gene related to that,the effect of Bu Shen Sheng Xue Yao (BSSXY, Traditional Chinese Medicine, ripe for tonifying the kidney to promote hematopiesis) on the level of granulocyte-macrophagocyte colony-stimulating factor (GM-CSF) gene transcription in marrow cells of aged mice was studied. Methods: After natural aged mice and D-gal's aging mice were medicated with BSSXY for two months and a month respectively,the transcription level of GM-CSF gene in marrow cells was measured by the techniques of semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR). Results: The transcription level of GM-CSF gene in the marrow cells of natural aged mice and D-gal's aging mice were significantly lower than those of young and normal control. After medication with BSSXY, they were obviously improved. Conclusions: One of the mechanisms of BSSXY's effects is to promote the transcription of GM-CSF gene. GM-CSF is one of the molecular basis of the theory that “ The kidney Produces the Marrow”. GM-CSF gene is one of the essential genes related to the theory above.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 28-32 )