A New Model of Cortical Devasculation for Vascular Dementia Research

doi:10.3969/j.issn.1006-3250.2001.02.022

Abstract：Objective:According to the pathology of VD,we develop a newanimal VD model wich is a rat model of cortical devasculation.Method:In this study,we used behavior,AchE histochemical and ChAT,BDNF,trkB immunohistochemical methods to discuss the expression of AchE,ChAT,BDNF,trkB after cortical devasculation.Result:This VD model with devasculation of rat left cortex cause damages in rat cortex and multiple layers and multiple structures beneath the cortex and behaviors.In behavior study,the achievement of leaning and memory of model rats decreased remarkably in the step down test and water maze.In HE and Nissl staining,there is an obvious destruction of cytoarchitecture and distribution of cells in cortex on the side of the lesion,shrinkage and vacuole of cells are found in cortex,especially in the cingulatic cortex.Shrinkage of cells is found in the pyramidal cell layer of CA1-CA4 subregions of hippocampus and the granule cell layer of dentate.This change is found obviously in the CA1 subregions.The Shrinkage of cells are also found in magnocllular basal nucleus(MBN),The quantity of AchE positive fibers decreased remarkably in the cortex,pyramidal cell layer of CA1-CA4 subregions of hippocampus and granule cell layer of dentate,especially in the cingulatic cortex and CA1 subregion,this change is more obvious.The quantity of BDNF,trkBpositive neurons decrease obviously in the cortex and hippocampus after cortical devasculation,Those changes are obvious in the cinbulatic cortex and CA1 subregion of hippocampus.The quantity of AchE,ChAT,BDNF,trkB positive neurons decrease obviously in the MBN,and the atrophic cells are also found in MBN,cell body become small,branches become less or even disappear.Conclusion:These results show that this model is an ideal model for the study of VD.