Effect of satragaloside Ⅳ on the enhance of endothelial permeability induced by histamine

doi:10.3969/j.issn.1006-3250.2001.02.018

Abstract：Objective:We studied effect of satragaloside Ⅳ on the increase ofendothelial permeability induced by histamine.Methods:The endothelial cells isolated from Bovine aorta were culture on polycarborate microporous filter membrane to develop confluent endothelial monolayer.Fluid filtration coefficient(Kf),filtration volume(Jv)and osmotic reflective coefficient(σ) to protein were measured in the endothelial monolayer treated by 10-4M histamine for 30 min or treated by 10－4M histamine for 30 min preincubated 10-4g.ml-1 satragaloside Ⅳ for 60 min after perfused either Hanks balanced salt solution or Hanks balanced salt solution containing 5 g.L-1 albumin.Results:The findings showed that Kf,Jv were increased after Perfused Hanks balanced salt solution,and Kf,Jv were increased and σ decreased after perfused Hanks balanced salt solution containing 5 g.L-1 albumin in the endothelial monolayer treated by 10-4M histamine.Application of satragaloside Ⅳ in the endothelial monolayer treated with histamine induced reducing of Kf,and Jv after perfused Hanks balanced salt solution,and induced reduce of Kf,and Jv and σ enhance after perfused Hanks balanced salt solution containing 5 g.L-1 albumin,compared with in the endothelial monolayer treated with histamine purely.Conclusions:The results indicated that increases in the endothelial momolayer permeability induced by histamine,and satragaloside Ⅳ can inhibit increases in the endothelial monolayer permeability induced by histamine.It is necessary that the cellular mechanism of permeability response induced by satragaloside Ⅳ remain to be further elucidated.