In vitro drug susceptibility analysis of 10 antifungal agents against Candida isolates from superficial candidiasis
LIU Mei
HE Wenjing
LI Lulu
TAN Jingwen
YANG Lianjuan
Abstract：Objective To investigate the species distribution and antifungal susceptibility profiles of superficial Candida infections in Shanghai Skin Disease Hospital.Methods A total of 144 Candida isolates were collected from clinical specimens(including groin,nails,and genitalia)between November 2024 and April 2025.Species identification was performed using ITS(internal transcribed spacer)sequencing.Antifungal susceptibility testing was conducted in accordance with the CLSI M27-A4 guideline.Results Of the 144 pathogenic isolates,Candida albicans(54.2%)and Candida parapsilosis(33.3%)were the predominant species.C.albicans exhibited high susceptibility to echinocandins and amphotericin B,but showed a resistance rate of 12.82%to fluconazole.C.parapsilosis showed high susceptibility to most antifungal drugs,with 100%susceptibility to echinocandins,voriconazole,and itraconazole,and the 89.58%susceptibility to fluconazole.Candida tropical was susceptible to echinocandins and amphotericin B,but showed a significant resistance to azole drugs.All C.tropical isolates exhibited reduced susceptibility(non-wild-type)to posaconazole,with one isolate resistant to fluconazole and one to voriconazole.Meyerozyma guilliermondii,Candida metapsilosis and Meyerozyma caribbica were mostly susceptible to antifungal drugs,with less resistance observed.Most strains of C.haemulonii,C.dermatis,Wickerhamomyces anomalus,and Debaryomyces hansenii also exhibited low MIC values across antifungal drugs tested.However,C.haemulonii showed a significant resistance tendency to fluconazole(with MIC 128 μg/mL)and terbinafine(64 μg/mL).C.dermatis had elevated MICs to echinocandins(8 μg/mL)and 5-fluorocytosine(16 μg/mL).Conclusion C.albicans and C.parapsilosis are the leading pathogens in superficial candidiasis.The significant interspecies differences in antifungal susceptibility observed necessitates species-level identification and ongoing resistance surveillance to guide effective individualized therapy.
Keywords：superficial fungal infectionsCandida drug sensitivityfungal drug sensitivity analysisdistribution of Candida species
Publication Date：2025-12-28
Online Publishing Date：2026-01-06(First online date of this platform, not the publication date of the document)
Pages：6( 658-663 )