Isolation, Identification and In Vitro Antifungal Susceptibility Analysis of Clinical Dermatophytes
Abstract：Objective To identify clinical isolates of dermatophytes and determine their antifungal susceptibility, providing a basis for the diagnosis and clinical treatment of dermatophytosis. Methods Clinical samples from patients with positive fungal microscopy were collected, and fungi were isolated and cultured. The obtained strains were identified by morphological and molecular biological methods, and antifungal susceptibility testing was performed to clarify the classification and drug sensitivity of the strains. Results Through morphological and molecular biological identification, all five skin dermatophyte isolates were identified as Trichophyton rubrum. They grew rapidly on Sabouraud glucose agar and potato dextrose agar, and sporulation was favored on oat agar and V8 agar. In vitro antifungal susceptibility testing determined the minimum inhibitory concentration (MIC) of seven antifungal agents against the isolated strains. The results showed that the geometric mean MIC of terbinafine was the lowest at 0.006 μg/mL, followed by voriconazole (0.012 μg/mL) and ketoconazole (0.027 μg/mL). Conclusion Five strains of Trichophyton rubrum isolated from different infection times, sites, and patients showed differences in growth, sporulation, and pigment production phenotypes, and none exhibited significant resistance to the seven common antifungal drugs.
Keywords：Trichophyton rubrumPhenotypeAntifungal susceptibility
Publication Date：2025-06-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 275-279 )