Evaluation of the clinical applicability of colloidal gold immunochromatography for the detection of derma-tophytes
ZANG Xiaohui
ZHANG Shuwen
LIU Weida
Abstract：Objective To evaluate the clinical applicability of colloidal gold immunochromatography in the diagnosis of der-matomycosis infection compared with fungal microscopic examination.Methods A total of 346 samples of skin or nail-scraps suspected of superficial fungal infection were collected from the Dermatology Hospital of Chinese Academy of Medical Sciences.The samples were divided into 3 parts,and fungal microscopic examination and immunochromatography antigen detection were performed,respectively.PCR was performed in cases when the results of the two methods were inconsistent.The consistency rate and inconsistency rate of the two methods were compared.Results The positive rate of antigen detec-tion and fungal microscopic examination is 76.59% and 69.36% ,respectively.Among 346 clinical samples,293 cases are consistent between two methods,and the consistency rate was 84.7% .The results of 53 cases were inconsistent between the two methods,and the inconsistency rate was 15.3% .46 of the incongruent clinical samples were further identified by PCR,of which 39 cases and 7 cases were identical with antigen detection and direct microscopic examination,respectively.That is,332 samples were consistent with the direct microscopic examination results(positive or negative)after PCR correction,with a consistent rate of 96.0% and an inconsistent rate of 4.0% .Conclusion Immunochromatography is a simple and reliable method for fungal detection.It is more sensitive than fungal microscopic examination and has good clinical value.
Keywords：dermatomycosiscolloidal gold immunochromatographyfungal microscopic examination
Publication Date：2024-04-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 109-112 )