Preliminary analysis of strains distribution,drug susceptibility characteristics and resistance gene of invasive Candida infections in a hospital in Xinjiang
ZHANG Songdi
WANG Xiaodong
AIKEDAI Yusufu
HADILIYA Hasimu
PALIDA Abulizi
Abstract：Objective To investigate the distribution of strains,drug susceptibility characteristics,and azole resistance mechanisms of invasive Candida infections in a hospital in Xinjiang.Methods 125 strains isolated from clinically diagnosed invasive fungal infections patients were identified by fungal morphology and molecμlar biology to analyse the distribution of Candida isolates,the susceptibility characteristics of 9 antifungal drugs,and sequencing of ERG11 and PDR1 gene in azole-resistant/non-wide-type(NWT)Candida isolates.Results Of the 111 yeast strains successfully identified,the top three were Candida albicans(50.45%),Candida glabrata(18.02%)and Candida tropicalis(15.32%).Most fungal isolates were susceptible to amphotericin B,5-fluorocytosine and echinocandins,and only two strains of C.glabrata were resistant to echinocandins.Azoles had good in vitro susceptibility to C.albicans,while the antifungal activities of posaconazole and voriconazole against C.glabrata and C.tropicalis were low(＜60%).Missense mutations of ERG11 gene was found in 9 of 33 strains of azole-resistant Candida strains(6 C.albicans strains,14 C.glabra strains,11 C.tropicalis strains,1 C.krusei strain and 1 C.parapsilosis strain),and missense mutations of PDR1 gene was found in all azole-resistant C.gla-bra.strains.Conclusion C.albicans is the predominant causative agent of invasive Candida infections.Echinocandins,amphotericin B,and 5-FC show good in vitro antifungal activity against Candida isolates,and azoles are less effective a-gainst C.glabrata and C.tropicalis;azole resistance may be associated with the mutations of ERG11 and PDR1 gene.
Keywords：Candidastrains distributiondrug susceptibility characteristicsERG11 genePDR1 genemutation
Publication Date：2023-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 493-500 )