Application of peripheral blood metagenomic next generation sequencing in the diagnosis of invasive pulmo-nary aspergillosis
DING Zhirong
ZENG Haiwen
LIN Tianlai
CHEN Weiwen
Abstract：Objective Metagenomic next generation sequencing was used to identify invasive pulmonary aspergillosis,which proved its important role in the clinical diagnosis of invasive pulmonary aspergillosis.Methods Three cases of inva-sive pulmonary aspergillosis were identified and diagnosed by peripheral blood metagenomic next generation sequencing in the Department of critical care medicine.The feasibility of peripheral blood metagenomic next generation sequencing in the diag-nosis of invasive pulmonary aspergillosis was analyzed and summarized.Results Of the three cases,case 1 had no underly-ing disease,case 2 had chronic obstructive pulmonary disease,and case 3 had T-lymphoblastic lymphoma/leukemia with a-granulocytosis after chemotherapy.Invasive pulmonary aspergillosis was diagnosed by bronchoalveolar lavage fluid(BALF)culture,sputum culture,peripheral blood metagenomic next generation sequencing,blood G test,GM test and other meth-ods.Conclusions Detection of Aspergillus cfDNA by peripheral blood metagenomic next generation sequencing,which is a rapid,and simple diagnostic method,can be used for the diagnosis of invasive pulmonary aspergillosis.
Keywords：invasive pulmonary aspergillosisdiagnosisperipheral bloodmetagenomic next generation sequencing
Publication Date：2023-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 411-415,422 )