In vitro chessboard detection for anti-Cryptococcuseffect of rattlerin and fluconazole
LEI Yan
FANG Wei
ZHANG Chao
LIAO Wan-qing
Abstract：Objective To determine whether there is synergistic effect of rattlerin combined with fluconazole against Cryptococcus in vitro by the checkerboard method.Methods According to the microdilution method(M27-A3),the fluconazole and rattlerin single drug susceptibility test[FLZ,RAT]and the Fractional Inhibitory Concentration Index(FICI)were used as the judgment indicators of combined drug susceptibility test[FLZ+RAT].Results The MIC values of rattlerin to 37 strains of Cryptococcus were 32-256μg/mL.When combined with fluconazole,FICI was≤0.50,which reduced the MIC value of fluconazole by 2-16times.Conclusion Rattlerin had a certain antifungal effect on Cryptococcus,and it could synergize with fluconazole to improve the sensitivity of fluconazole to Cryptococcus.
Keywords：rattlerinfluconazoleCryptococcuscheckerboard methodcombined drug sensitivity
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 90-94 )