Study on the mechanism of NT-89against Candida albicans based on quantitative proteomics
LIU Yu
YAN Lan
JIANG Yuan-ying
Abstract：Objective To analyze the differentially expressed proteins after the treatment of anti-fungal compound NT-89against Candida albicansby quantitative proteomics iTRAQ technology.Methods Total protein and cell wall proteins of C. albicans were extracted with or without NT-89treatment.The relative abundanceand differential proteins in protein extracts were detected by iTRAQ.Then the function classification of the differential proteins was performed using GO database.Results 295differential proteins were detected in extract of total protein(TP),and Ywp1p and Pga10p were most significantly down-regulated in total protein.6GPI-anchored proteins in cell wall protein(CWP)extract were found significantly downregulated. Conclusion NT-89disrupted the structural integrity and function of cell wall in C.albicans.The iTRAQ technology can provide effective reference information for drug mechanism research.
Keywords：NT-89Candida albicanscell wallHF-pyridineGPI-anchored proteiniTRAQ
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 70-77 )