Evaluate the accuracy of CFW fluorescent staining for the diagnosis of suspected onychomycosis
YUE Xue-ping
SHI Xiu-yan
CHEN Wei
WAN Zhe
LI Ruo-yu
WANG Ai-ping
LI Qing
Abstract：Objective Evaluate the positive rate,accuracy and efficiency of CFW (Calcofluor white) fluorescent staining for the diagnosis of suspected onychomycosis.Methods Outpatients suspected to have onychomycosis were recruited.Each patient was subjected to three methods (CFW fluorescent staining,KOH microscopy and fungal culture) together to confirm the diagnosis.Then we compared the positive rates and the statistically significant differences across the three methods.Moreover,the images under KOH and CFW were also compared.Results One hundred patients with 100 affected nails were recruited.Among them,the positive rates of the CFW,KOH,and culture were 64 ％,63 ％,and 31％,respectively.According to the McNemar test,CFW had a higher percentage of positive rate than culture testing (P＜0.01),but it was not statistically significant different from KOH (P＞0.05).Compared with KOH,the preparation and examination time were faster and easier,and the fungal structures under CFW were clearer,more obvious,and more easily detected.Conclusions CFW fluorescent staining was a simple,fast,convenient,accurate,and time-efficient method.It could potentially increase the accuracy and efficiency of the diagnosis of onychomycosis and help to avoid misdiagnosis.
Keywords：onychomycosisCFW fluorescent stainingKOH microscopyfungal culture
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 8-10,45 )