Butyl alcohol extract of BaiTouWeng decoction inhibit vulvovaginal candidiasis in murine model via down-regulating NLRP3 inflammasome
DUAN Qiang-jun
YUN yun
CHEN Zhi-zhen
SHAO Jing
WANG Ting-ming
WANG Chang-zhong
Abstract：Objective To study the effects of Butyl alcohol extract of BaiTouWeng decoction (BAEB) on vulvovaginal candidiasis (VVC) in murine model.Methods Seventy-two female mice were randomly divided into normal group,model group,BAEB high-dose group,BAEB middle-dose group,BAEB low-dose group,and fluconazole group.The mice were firstly injected with estradiol to induce oestrum,and then intravaginally administrated with 2 × 106 CFU/mL Candida albicans.After 7-day treatment,The fungal load (CFU/mL) was assessed from vaginal lavage fluid,the pathological changes of vaginal tissues were observed by HE staining.The levels of IL-1β and IL-18 in vaginal tissues were detected by ELISA.The mRNA expressions of NLRP3,ASC,caspase-1 were analyzed by qRT-PCR.Results Compared with the normal group,the vaginal mucosa of the model group was completely destroyed,and the infiltrations of plenty of inflammatory cells were observed.Compared with the model group,BAEB groups showed improvements to varying degrees.The levels of IL-1β and IL-18 in the model group were significantly higher than those in the normal group (P ＜ 0.001).The mRNA expressions of TLR2,TLR4,NL-RP3,ASC and caspase-1 were up-regulated significantly (P＜0.001).The levels of IL-1β and IL-18 in the BAEB groups were much lower than those in the control group (P＜0.001).Conclusion The BAEB could be used to treat the vulvovaginal candidiasis in mice.
Keywords：vulvovaginal candidiasis (VVC)NLRP3 inflammasomeButyl alcohol extract of BaiTouWeng (BAEB)Candida albicans
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 337-341 )