Effects of transforming growth factor on the function of macrophage against Cryptococcus neoformans
LI Ying-Fang
SANG Jun-Jun
LI Juan
JIANG Wei-Wei
LIAO Wan-Qing
PAN Wei-Hua
Abstract：Objective To investigate the phagocytosis and killing ability of macrophage under TGF-β intervention.Methods Using TGF-β intervene phagocytosis and killing assay of activated macrophages in vitro.Phagocytic index of macrophage and the intracellular growth of Cryptococcus neoformans was observed respectively.Nitric oxide (NO) production was detected using the Griess regent kit,and then comparing the changes of NO production in macrophages under the TGF-β intervention.Results The phagocytic index with the treatment of TGF-β decreased by 43.9 ％,significantly lower than control group (P＜ 0.05).While the production of NO increased and the intracellular fungal burden significantly declined under the intervention of TGF-β (P＜0.05).Conclusion Chemotaxis and phagocytosis of activated macrophage were inhibited under the intervention of TGF-β in vitro,but the NO production increased and killing ability enhanced.
Keywords：Cryptococcus neoformanstransforming growth factormacrophage
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 65-68 )