Construction and verification of stable down-regulated MMP9, S100A10 mouse brain microvascular endothelial cell lines
CHEN Xue-wen
ZHU Hong-mei
WEN Hai
Abstract：Objective To construct stable down-regulated MMP9,S100A10 mouse brain microvascular endothelial cell lines,then verify the reduction level of gene expression.Methods By transfecting LV-musMMP9-shRNA,LV-musS100A10-shRNA into b.End3 respectively,then the very cell lines were screened by puromycin.The obtained differential b.End3 cell lines were observed by fluorescence microscope and varified by RT-qPCR.Results The study successfully constructed stable transfected cell lines which could effectively interference S100A10,MMP-9 expression of 3 × 108 TU/mL and 1 × 108 TU/mL LV-musS100A10-shRNA respectively.Conclusions The targeted down-regulation b.End3 cell lines are useful for further exploring the role of molecule S100A10 or MMP-9 in host blood-brain barrier by control variables and the possible target of treatment exploration of cryptococcal encephalitis/meningitis.
Keywords：Lentivirus vectorMMP9S100A10b.End3
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 19-22,18 )