Effects of Aspergillus fumigatus spore on the activation of mast cell
Abstract：Objective To study the etfects of Aspergillus fumigatus spore on the activation of human mast cell line HMC-1.Methods Prepared heat-killed Aspergillusfumigatus spore antigen by heating method.HMC-1 cells were co-cultured with heat-killed Aspergil lus fumigatus spores whose concentration was 5 × 105.Microplate spectrophotometer was applied to the β-hexosaminidase release assay which was an index of mast cell degranulation.Enzyme-linked immunosorbent assay (ELISA) was used for released IL-5.The expression levels of IL-5 mRNA was detected by Realtime-PCR.Results Compared with the negative control group,heat-killed Aspergillus fumigatus spores increased β-hexosaminidase release [(11.3 ％ ± 1.2 ％) VS (0.5％±0.1％),P＜0.05],IL-5 mRNA expression level [(2.47±0.12) VS (0±0.26),P＜0.05] and the release of IL-5 [(11.96±1.09) VS (9±1.36),P＜0.05] in HMC-1 cells.Conclusion Aspergillusfumigatus spores activates mast cell line HMC-1 resulting in cell degranulation,synthesis and release of IL-5.
Keywords：Aspergillus fumigatussporemast celldegranulationinterleukin-5
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3-7 )