Effect of baicalin in combination with fluconazole against Candida albicans morphological transition from yeast to hyphae via inhibiting cAMP
FENG Xin
SHI Gao-xiang
YUN Yun
WU Da-qiang
SHAO Jing
WANG Tian-ming
WANG Chang-zhong
Abstract：Objective This study aimed to investigate the antifungal activity of baicalin in combination with fluconazole a-gainst Candida albicans morphological transition from yeast to hyphae and to explore the mechanism.Methods The hypha-inducing liquid and solid media were uesd to observe the effect of baicalin alone or in combination with fluconazole on yeast-hapal transformation in C .albicans ;The intracellular cAMP level and cAMP rescue was measured by ELISA Kit;Quantitative real time PCR (qRT-PCR)was performed to measure the expression of cAMP-related genes.Results Baicalin in combination with fluconazole could inhibit hyphae formation and intracellular cAMP level obviously;exogenous cAMP could convert hy-pha-inhibiting effect;And expression of RAS1 and CDC35 were downregulated by 35% and 27% respectively,while the ex-pression of PDE2 was upregulated by 121%.Conclusion Mechanism of baicalin in combination with fluconazole inhibiting C . albicans morphological transition was probabaly via down-regulating the intracellular cAMP.
Keywords：C .albicansbaicalinfluconazolemorphological transitioncAMP
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 8-12 )