Construction and identification of Candida albicans FLO8 mutations
LI Wen-jing
LIU Ming
LIU Jin-yan
SHI Ce
WANG Ying
ZHAO Yue
XIANG Ming-jie
Abstract：Objective To construct Candida albicans FLO8 mutations.Methods We inserted Candida albicans FLO8 gene under the control of the ADH1 promoter in pCP20 plasmid,and plasmids containing the point mutations FLO8 R209T , FLO8 A3 1 1 T ,FLO8 6 54Ter ,FLO8 G723R and FLO8 T7 5 1 D were constructed using site-directed mutagenesis from plasmid pCP20.Then we introduced the inserts with FLO8 point mutations into Candida albicans SN1 52 flo8/flo8 at the endogenous ADE2 gene loci by homologous recombination.Results The plasmids with FLO8 point mutations were constructed successfully by DNA sequencing;The inserts with FLO8 point mutations were exactly recombined to the ADE2 gene loci of Candida albicans SN1 52 flo8/flo8 as confirmed by PCR.Conclusions Candida albicans FLO8 mutations can be efficiently constructed with pCP20 plasmid by site-directed mutagenesis,homologous recombination,etc.
Keywords：Candida albicansFLO8mutations
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1-7 )