Protective effect of thioredoxin reductase GliT of Aspergillus fumigatus in a murine model of invasive aspergillosis
LIU Qian
SHI Li-ning
SHANG Xiu-juan
CHEN Xiao
HU Yu-an
CHEN Yong
LI Fang-qiu
Abstract：Objective To investigate the immunoprotection in mice immunized with thioredoxin reductase GliT of Asper-gillus fumigatus .Methods C57BL/6 mice were randomly assigned into two groups:immunization group intramuscularly im-munized with recombinant TR for two times;control group intramuscularly immunized with PBS instead of TR.Survival rate, A.fumigatus lung burdens,pathologic examination and cell count of alveolar lavage were evaluated.Results The survival rate of immunization group was 64.7% within 7 days after infection while that of control group was 0.The medians of CFUs from the lung tissues of the suvivors and the nonsuvivors were 0 and 44 (P 25 ,P 7 5 were 21,70)respectively (P <0.01).Pulmonary pathology indicated that the lungs of the dead immunized mice and control mice showed alveolar hemorrhage,inflammatory cells recruitment,focal tissue necrosis and hyphal elements throughout the entire lung.In contrast,the lung tissues of the sur-vivors were damaged slightly and free of hyphal elements.Alveolar lavage fluid smear showed that the counting of the survival is given priority to with mononuclear cells,accounting for about 84.2%,neutrophils accounted 1 5.8%.The counting of the nonsurvival is given priority to with neutrophils,accounting for about 66.4%,while 33.6% mononuclear cells.Conclusion Re-combinant TR of Aspergillus fumigatus can induce a modest protection in a murine model of invasive aspergillosis and might be a potential protective antigen.
Keywords：Aspergillus fumigatusthioredoxin reductase GliTprotective effect
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 340-344 )