Identification and knocking-out of polarized growth factor gene kipA in Aspergillus fumigates
ZHU Jun-hao
LI Li
ZHANG Qiang-qiang
CHEN Yu-chong
Abstract：Objective To construct the kipA mutant strain.Methods The vector containing upper and downstream genes of kipA linked with hygromycin resistance gene in the middle was constrctied via In-Fusion assembly.The kipA gene of As-pergillus fumigatu was replaced with hygromycin resistance gene via the method of homologous recombination.The mutant strain was identified and validated by realtime-PCR.Results The kipA deletion strains were successfully constructed.Conclu-sions Successful construction of kipA mutant strain would provide the foundation for the research of polarized growth and vascular invasion of Aspergillus fumigatus .
Keywords：Aspergillus fumigatuskipA genepolarized growth
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 332-335 )