The inhibitory effects of Butyl alcohol extract of BaiTouWeng decoction on biofilm formation of Candida albicans

ZHANG Meng-xiang; LU Ke-qiao; XIA Dan; XIA Xue; SHI Gao-xiang; SHAO Jing; WU Da-qiang; WANG Tian-ming; WANG Chang-zhong

doi:10.3969/j.issn.1673-3827.2015.06.001

Abstract：Objective To investigate the effects of Butyl alcohol extract of BaiTouWeng decoction (BAEB)on the biofilm formation of Candida albicans clinical strains isolated from vulvovaginal Candidiasis (VVC).Methods Microdilution meth-ods was used to determine the MIC.XTT reduction assay was applied to determine the SMIC80 .Time-kill curve method was applied to detect the effects of BAEB on living cells of Candida albicans .Crystal violet staining method was used to determine the biomass of the biofilm.Scanning electron microscopy (SEM)was applied to observe the morphological changes of the bio-film.Confocal laser scanning microscopy (CLSM)was applied to determine the thickness of the biofilm.The quantification re-al-time PCR (qRT-PCR)was used to detect expression changes of genes (HSP90 ,UME6 and PES1 )of the biofilm treated by BAEB.Results The MICs of BAEB against C .albicans strains are determined as 64~256 μg/mL.The SMIC80 s of BAEB against the biofilm of C .albicans strains are determined as ≥1 024 μg/mL.Time-kill curve results indicate that BAEB has a promise antifungal effect at concentrations of 5 12 and 1 024 μg/mL.Crystal violet staining results show that the biomass of C .albicans is reduced by BAEB at 5 12 and 1 024 μg/mL.SEM results indicate that the formation of C .albicans biofilm carriers is inhibited by BAEB on different adhesion,and the morphol-ogy of biofilm is also affected by BAEB.The thickness of C .albicans biofilm is reduced by BAEB accord-ing to CLSM results.Furthermore,qRT-PCR results indicate that expression of UME6 is significantly down-regulated by BAEB at 256,5 12,1 024 μg/mL,and HSP90 is up-regulated at 5 12 and 1 024 μg/mL of BAEB,and PES1 is not affected by BAEB at any concentration.Conclusion BAEB inhibits effectively the biofilm formation of VVC strains of C . albicans .