MLPA application in clinical diagnosis of pathogen
FANG Wen?jie
LIU Jia
PAN Wei?hua
LIAO Wan?qing
Abstract：As for patients diagnosed with Infectious diseases,favorable prognosis is determined by correct diagnosis and proper treatment.So it is important to diagnose clinical pathogens rapidly.Compared with other clinical test methods,MLPA is a simple,val?id,and fast one which can detect several gene sequences in quantity at the same time. The steps include:the degeneration of DNA sample,hybridization,ligation,and PCR amplification.Although being at its first stage,there are already certain applications of MLPA in diagnosing and researching of clinic microorganism,and the peculiarity of easy operation,fast reaction,high pass rate and high sen?sitivity have reveal bright prospect of MLPA.
Keywords：multiplex ligation?dependent probe amplificationpathogendiagnosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 307-311 )