Species identification of Sporothrix clinical isolates in Northern and Southern of China
HE Yu
HUANG Meng?Ya
HU Qing?bi
ZHOU Xun
Abstract：Objective To identify 50 Sporothrix clinical isolates in Northern and Southern of China.Methods All isolates from patients were subcultured at 25℃ and morphological characteristic was analysed by microscope and visual inspection. The total ge?nomic DNA was extracted to amplify the partialβ?tubulin gene and the internal transcribed ( ITS) region by polymerase chain reac?tion (PCR).The phylogenetic tree was constructed by DNA sequences analyses based on datasets of ITS and a combined ITS and partial β?tubulin region using maxium likehood (ML) and neighbor?joining (NJ) methods with MEGA5 software.The Reference se?quences used for phylogenetic analyses were retrieved from GenBank. Result The macroscopic morphologies and microscopic fea?tures of all isolates were comformed to Sporothrix spp.The phylogenetic tree showed that all of the isolates were clustered in a distinct clade with a type of Sporothrix globosa.Conclusion S.globosa may be the major Sporothrix existing in northern and southern of Chi?na,which can provide basis for the epidemiological investigation of Sporothrix.
Keywords：SporothrixmorphologyphylogenySporothrix globosa
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 279-282 )