The effects of T.asahii on monocyte?derived DCs phenotypic and function
WANG Rui?Li
AO Jun?hong
LIAO Yong
YANG Rong?ya
YANG Dong?qian
LV Xue?lian
CHEN Shan?shan
Abstract：Objective To investigate the effects of Trichosporon asahii on the phenotypic and functional maturation of human pe?ripheral blood monocyte?derived dendritic cells (DCs).Methods The monocyte?derived DCs of health donors were obtained by cul?ture in vitro.In the experimental groups,DCs stimulated by different doses of heat?inactived T.asahii conidia (DCs : T.asahii=1 : 1, 1 : 5,1 : 10) ,RPMI?1640 and lipopolysaccharide ( LPS) stimulation groups were as the blank control and positive control,respec?tively.Shapes of the cells were observed under the inverted microscope,the rate of phagocytosis was calculated by Wright Giemsa stai?ning.DCs maturation was analysed by using flow cytometry.Results The morphology of DCs was significantly changed during incuba?tion.At 24 h,more than one T.asahii conidias were efficiently bound and internalized by DCs and the phagocytic rates were signifi?cantly different between the experimental groups (P<0.05).The expressions of CD80,CD86,CD83 in the experimental groups were much higher than the blank control group (P<0.05),but significantly lower than LPS group (P<0.05).Conclusion T.asahii can change the morphology of DCs and promote the expression of surface molecules,which enhance the maturation of the dendritic cells.
Keywords：T.asahiidendritic cellssurface molecules
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 266-271 )