Identification and knocking-out of transcription co-activation factor gene MBF1 in Cryptococcus neoformans
ZHOU Zhaojing
MENG Yunfang
ZHAO Jingyu
SANG Junjun
ZHANG Chao
FA Zhenzong
FANG Wei
LIAO Wanqing
Abstract：Objective To construct the mutant strain of transcriptional co?activated factor Mbf1 in Cryptococcus neoformans . Methods Amplified the homologous recombination cassette containing report gene NEO and flanking DNA fragments of MBF1 via overlap PCR,which was then transformed into Cryptococcus neoformans through gene gun.The positive transformants was screened by diagnostic PCR and then confirmed by DNA sequencing.Results The target gene MBF1 was successfully knocked out in Cryptococcus neoformans.Conclusion Construction of the mutant strain would lay the foundation for further pathogenetic studies research of tran?scriptional co?activated factor Mbf1 in Cryptococcus neoformans.
Keywords：Cryptococcus neoformansPathogenesisGene Knocking-out
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 80-83 )