Analysis of relationship between transcription factor RPN4 and drug susceptibility of Candida albicans
XU Hongtao
TANG Ruoyu
QU Yingge
WANG Xiaojuan
JIANG Yuanying
CAO Yongbing
YAN Tianhua
Abstract：Objective To study the influence on the drug susceptibility of Candida albicans after transcription factor RPN4 was knocked out and the mechanism how this transcription factor regulates the drug susceptibility in Candida albicans.Methods The broth microdilution method was used to determine the minimal inhibitory concentrations (MICs) of 30 strains transcription factor knocked out Candida albicans .The drug susceptibility was reassured by spot assay and growth curve.The total RNA of parental strain SN250 and susceptible strains were extracted and the expression of multidrug resistance genes CDR1 and MDR1 was determined by real?time PCR,and the pump ability was examined by the ability of pump out rhodamine 6G.Results Compaired to the parental strain SN250,transcription factor RPN4 knocked out strain was more susceptible to fluconazole.The expression of CDR1 and MDR1 was lower than SN250 and the pump ability was also weaker than SN250.Conclusion The knockout of transcription factor PRN4 re?sults susceptible to fluconazole in Candida albicans,and this transcription factor may be involved in the regulation of drug susceptibil?ity in Candida albicans.
Keywords：Candida albicanstranscription factorsdrug susceptibilitymultidrug resistance genes
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 75-79 )