Anti-adhesion effect of Butyl alcohol extract of BaiTouWeng decoction on Candida albicans isolated from VVC
Abstract：Objective To investigate anti?adhesion effect of Butyl alcohol extract of BaiTouWeng decoction ( BAEB) on Candida albicans.Methods Agar plate assay was used to determine CFU of Candida albicans for 2h and 4h.XTT reduction assay was applied to evaluate the adherent Candida albicans merabolic activity.Test tube assay was utilized to evaluate flocculation.The water?hydrocar?bon two?phase assay was employed to measure the cell surface hydrophobicity of Candida albicans . Scanning electron microscope (SEM)and fluorescein diacetate(FDA)staining was used to observe the morphological changes of adherent Candida albicans .qRT?PCR was adopted to inspect the expression of adhesion?related genes such as INT1, ALS3, MNT2, ALA1, ALS1, ALS5, HWP1 and CSH1.Results 512,1 024μg/mL BAEB could effectively inhibit adherence of Candida albicans .Flocculation was delayed by 512, 1 024 μg/mL BAEB.The cell surface hydrophobicity in the BAEB treated groups was lower than that of the control group.The expres?sion of ALS1,CSH1,ALA1,ALS3,INT1,HWP1 were downregulated dramatically after BAEB treatment,but ALS5 and MNT2 were un?changed.Conclusion BAEB could effectively inhibit adherence of Candida albicans isolated from VVC by regulating adhesion?asso?ciated genes.
Keywords：butyl alcohol extract of BaiTouWeng decoction ( BAEB)vulvovaginal candidiasis( VVC)Candida albicansadhe-sion
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 25-29,30 )