Study of improved colony PCR technologies for the identification of Trichosporon asahii
ZHANG Dequan
LIU Han
LIAO Yong
XIA Zhikuan
YANG Dongqian
YANG Yang
LV Xuelian
YANG Rongya
Abstract：Objective To explore a direct colony PCR method for rapid identification of T.asahii.Methods Four methods inclu?ding direct colony,direct colony with centrifugation,enzymolysis,and improved enzymolysis method were used to preliminarily sample treatment.The sensitivity and the positive rate of colony PCR based on four methods for isolates of T.asahii were evaluated and com?pared with the PCR results of the traditional DNA extraction.Results The sensitivity of the improved enzymolysis method,direct col?ony method and the traditional DNA extraction were both 102 CFU/mL,while the positive rates were 93.75%,75% and 50%,respec?tively.The direct colony with centrifugation presented the sensitivity of 104 CFU/mL and the positive rate of 43.75%.The enzymolysis method presented total negative results in the detection of both sensitivity and positive rate. Conclusion The improved enzymolysis method which presented the advantages of high sensitivity and positive rate of trace samples,could be applied for the rapid identifica?tion of T.asahii.
Keywords：Colony PCRTrichosporon asahiiDNA preliminary extraction
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 6-10 )