Using LC-MS/MS for determination of Gliotoxin in vitro model of human bronchial epithelial infected by Aspergillus fumigatus
SHEN Liang-liang
Han yu
Zheng Jian-feng
Zhao Jing-jun
Abstract：Objective To establish a LC-MS/MS method for the in vitro determination of Gliotoxin( GT)in co-culture model of human bronchial epithelial( HBE)infected by Aspergillus fumigatus( AF) Methods Developing co-cultured model of HBE infec-ted by AF,the concentration of GT were detected by LC-MS/MS in different periods Results The GT concentration of supernatant from co-culture increased over time( P <0 05)and the recovery of GT was 68 7%-72 6% Conclusion This method is sensi-tive,simple and accurate,and suitable for the determination of GT in the co-culture model.
Keywords：human bronchial epithelial cellAspergillus fumigatusglioxinLC-MS/MSdetermination
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 268-271 )