MLST genotyping and mating type identification of C. neoformans in genotypes with virulent difference
KANG Ying-qian
CHEN Yu-ru
WANG Mei-zhu
ZHAO Liang
LI Xiao-ling
JIN Fang
LIU Tao-hua
Abstract：Objective To study the Multilocus Sequence Typing( MLST)characteristics of C. neoformans var grubii and to i-dentify their mating types Methods Primers of 7 housekeeping genes(CAP59,GPD1,LAC1,PLB1,SOD1,URA5 and IGS1)were designed for PCR amplification;the genotypes of 20 environment and clinical isolates were analyzed with MLST result and the mat-ting types of these strains were identified And both the results of MLST and Multilocus Microsatellite Typing( MLMT)were com-pared to each other for evaluating their stability and reliability in taxonomy Results The multilocus microsatellite genotype of all the 10 environment isolates was MLMT-13 in previous experiment and the MLST type of there strains was ST-15 in this experiment Sim-ilarly,all the MLMT-36 clinical isolates belong to ST-32 And all the strains were MAT-α Conclusion The MLST results are high-ly consistent with MLMT results in previous experiment which reveals that both molecular typing methods are high resoluted and sta-ble on classification of different isolation background on species or varieties level.
Keywords：Cryptococcus neoformanceMLSTMLMTmating type
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 264-267 )