Synergistic effect of baicalein in combination with fluconazole on Candida albicans biofilm
ZHAO Liu-ya
JIANG Jing-chen
YAO Xiang-wen
CAO Ying-ying
JIANG Yuan-ying
Abstract：Objective To investigate the effect of baicalein(BE)in combination with fluconazole(FLC)on Candida albicans biofilm formation. Methods The inhibition of C. albicans biofilm by BE in combination with FLC was determined by confocal scan-ning laser microscopy and XTT methods. The water-hydrocarbon two-phase assay was used to measure the cell surface hydrophobicity (CSH). The expression of CSH1,EFG1,HWP1,ALS1 mRNA was measured by Real time RT-PCR. Results BE in combination with FLC could inhibit the formation of C. albicans biofilm. The cell surface hydrophobicity in the BE and FLC-treated group was lower than that in the groups treated by BE or FLC alone. Consistent with this,BE and FLC-treated biofilm cells expressed lower lev-els of CSH1,EFG1,HWP1 mRNA than the cells grown in the presence of BE or FLC alone. Conclusions BE in combination with FLC could inhibit the formation of C. albicans biofilm.
Keywords：Candida albicansbaicaleinfluconazolebiofilm
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 70-74 )