LncRNA TTN-AS1 regulates the miR-320a/CPEB1 signaling axis to affect the growth and metastasis of osteosarcoma cells
LIU Guangfei
GUO Zhiyuan
WANG Yanhua
LU Shouliang
GUO Lei
CHENG Cai
Abstract：Objective To investigate the mechanism of long non-coding RNA(lncRNA)TTN-AS1 in regulating the biological behavior of osteosarcoma cells.Methods A total of fifty patients with osteosarcoma who were surgically treated in Cangzhou Central Hospital from February 2022 to October 2024 were collected,and the expression of TTN-AS1 in osteosarcoma and adjacent tissues was detected by qRT-PCR.Osteosarcoma Hos cells were divided into Vector group,TTN-AS1 group,TTN-AS1+miR-320a mimic group,and TTN-AS1+miR-320a mimic+CPEB1 group.143B cells were divided into Control group,sh-TTN-AS1 group,sh-TTN-AS1+miR-320a inhibitor group,and sh-TTN-AS1+miR-320a inhibitor+si-CPEB1 group.The expression level of TTN-AS1 in osteosarcoma cell lines was detected by qRT-PCR.The dual luciferase reporter gene experiment verified the targeting relationship between TTN-AS1 and miR-320a,as well as the targeting relationship between miR-320a and CPEB1.CCK8 assay and clone formation assay were used to detect cell proliferation ability,flow cytometry was used to detect cell apoptosis,and Transwell assay was used to detect cell migration and invasion ability.Results TTN-AS1 was highly expressed in both osteosarcoma tissues and cells(P<0.05).MiR-320a was the target gene of TTN-AS1,and CPEB1 was the target gene of miR-320a.Compared with the Vector group,the cell proliferation,migration and invasion abilities of Hos cells in the TTN-AS1 group were significantly increased(P<0.05),while the apoptosis rate was significantly decreased(P<0.05).Compared with the TTN-AS1 group,the cell proliferation,migration and invasion abilities of Hos cells in the TTN-AS1+miR-320a mimic group were significantly decreased(P<0.05),while the apoptosis rate was significantly increased(P<0.05).Compared with the TTN-AS1+miR-320a mimic group,the proliferation,migration and invasion abilities of Hos cells in the TTN-AS1+miR-320a mimic+CPEB1 group were significantly increased(P<0.05),while the apoptosis rate significantly decreased(P<0.001).Compared with the Control group,the cell proliferation,migration and invasion abilities of 143B cells in the sh-TTN-AS1 group were significantly decreased(P<0.05),while the apoptosis rate was significantly increased(P<0.001).Compared with the sh-TTN-AS1 group,the cell proliferation,migration and invasion abilities of 143B cells in the sh-TTN-AS1+miR-320a inhibitor group were significantly increased(P<0.05),while the apoptosis rate significantly decreased(P<0.001).Compared with the sh-TTN-AS1+miR-320a inhibitor group,the cell proliferation,migration,and invasion abilities of 143B cells in the sh-TTN-AS1+miR-320a inhibitor+si-CPEB group were significantly decreased(P<0.05),while the apoptosis rate significantly increased(P<0.001).Conclusions TTN-AS1 is up-regulated in osteosarcoma tissues and cells,and it affects the biological behavior of osteosarcoma cells by regulating the miR-320a/CPEB1 pathway.
Keywords：OsteosarcomaTTN-AS1miR-320aCPEB1ProliferationApoptosisInvasionMigration
Publication Date：2026-02-20
Online Publishing Date：2026-03-09(First online date of this platform, not the publication date of the document)
Pages：8( 64-71 )