Study on the expression level of ADGRD1,PTK7 in non-small cell lung cancer and its relationship with lymph node metastasis and prognosis

LIU Zhuo; ZHAO Zheng; ZHANG Liangliang; YANG Cuiying; JIN Zhao; ZHOU Shujie

doi:10.3969/j.issn.1674-4136.2024.06.012

Abstract：Objective To investigate the expression levels of adhesion G protein coupled receptor D1 ( ADGRD1) and protein tyrosine kinase 7 ( PTK7) in non-small cell lung cancer ( NSCLC) tissues and their relationship with lymph node metastasis and prognosis.Methods One hundred and fifty-two NSCLC patients who were admitted to Handan Central Hospital from August 2019 to August 2020 were selected,and cancer tissues and cancer normal peripheral lung tissue ( distance from tumor margin≥2 cm) were collected.ADGRD1 and PTK7 expression levels in cancer tissues and cancer normal peripheral lung tissue were detected by immunohistochemical technique.The relationship between ADGRD1 expression,PTK7 expression and the clinicopathological characteristics of NSCLC patients were analyzed.The correlation between ADGRD1 expression and PTK7 expression in cancer tissues and lymph node metastasis were analyzed by Spearman test.The influencing factors of lymph node metastasis in NSCLC patients were analyzed by multivariate Logistic regression model.The correlation between ADGRD1 expression and PTK7 expression in cancer tissues were analyzed by Spearman test.After 3 years of follow-up,the prognosis of NSCLC patients was analyzed by Cox regression model and Kaplan-Meier survival curve.Results Compared with cancer normal peripheral lung tissue,ADGRD1 positive expression rate in cancer tissues was significantly decreased (P<0.05),and PTK7 positive expression rate was significantly increased ( P<0.05 ) .ADGRD1 positive expression rate in patients with lymph node metastasis,poorly differentiated and TNM stage Ⅲa NSCLC was significantly lower than that in patients with no lymph node metastasis,middle to highly differentiated and moderately and TNM stage Ⅰ+Ⅱ NSCLC ( P<0.05) .PTK7 positive expression rate in patients with lymph node metastasis and TNM stage Ⅲa was higher than that in patients with no lymph node metastasis and TNM stage Ⅰ+Ⅱ( P<0.05 ) .When ADGRD1 was negative and PTK7 was positive,the lymph node metastasis of NSCLC patients was significantly increased ( P<0.05) .PTK7 positive expression and ADGRD1 negative expression were risk factors for lymph node metastasis in NSCLC ( P<0.05 ) .Of the 152 cases,146 were finally followed up.The 3-year overall survival rate in ADGRD1 negative expression group was significantly lower than that in ADGRD1 positive expression group ( P<0.05 ) .The 3-year overall survival rate in PTK7 positive expression group was significantly lower than that in PTK7 negative expression group ( P<0.05) .Lymph node metastasis,ADGRD1 negative expression,TNM stage Ⅲa and PTK7 positive expression were risk factors for poor prognosis of NSCLC ( P<0.05) .ADGRD1 expression level in cancer tissues was negatively correlated with PTK7 expression level (P<0.05).Conclusions Low expression of ADGRD1 and high expression of PTK7 in cancer tissues of NSCLC patients are related to lymph node metastasis and poor prognosis.