Effect of ganoderma applanatum polysaccharides on human gastric carcinoma Cell SGC-7901,anti-pro-liferation and exprssion of CyclinD1,p16

JI Aijun; LU Jianwei; LI Suping; JIN Changwen

doi:10.3969/j.issn.1674-4136.2015.06.004

Abstract：Objective To investigate the effects of Ganoderma applanatum polysaccharides(GAPS) on pro-liferation and apoptosis of the human gastric carcinoma cell line 7901 and its possible mechanism of action on CyclinD1,p16. Methods The growth inhibition effect of GAPS on human gastric carcinoma cell line 7901 was observed by using MTT assay, and cell apoptosis was analyzed by flow cytometry. The expression of cy-clinD1mRNA and p16mRNA in SGC-7901 cells were analyzed by real-time fluorescence quantitative PCR. Re-sults The SGC-7901 cells were treated with various concentrations of GAPS for 48 h and 72 h, cell viability was determined by Alamar Blue assay. Cell growth was suppressed by GAPS of different Concentrations(0, 0. 125 mg/ ml,0. 25 mg/ ml, 0. 5 mg/ ml,1. 0 mg/ ml), GAPS inhibited the growth of gastric cancer cells in a dose-dependent manner. The cells were treated with various concentrations (0,0. 25 mg/ ml, 0. 5 mg/ ml,1. 0 mg/ ml) of GAPS for 72 h. The results showed that GAPS were capable of inducing an increase in the percenta-ges of G1-phase cells and the number of apoptotic cells (P < 0. 05). Real-time quantitative PCR was used to detect the mRNA expression of CyclinD1 and p16 at 72 h after GAPS treatment. The results revealed a signifi-cant down-regulation of mRNA expression of CyclinD1, and up-regulation of the mRNA expression levels of p16 as compared with the controls. Conclusions The results show that GAPS has significant action of anti-prolifera-tion and pro-apoptosis on human gastric carcinoma cell line 7901. The down-regulation of CyclinD1 suppresses the cell proliferation inhibition and promotes the cell apoptosis. p16 showed high expression, which can activate the negative feedback of cell cycle. The results suggest that GAPS may have an effect on the pathway of gastric cancer genesis,it prevents the cells from G1 / G0 phase to S phase.