Effects of siRNA silencing Livin gene expression on the suppression of hepatocellular carcinoma growth in nude mice
LI Qing
Abstract：Objective To study the effect of inhibitor of apoptosis Livin down-regulation by small interfer-ing RNA-mediated RNA interference (RNAi) in a xenograft model of hepatocellular carcinoma cell line MH-CC97H. Methods MHCC97H cells were transfected with synthetic small interfering RNA (siRNA) targeting Livin by lipidosome LipofectamineTM2000. Expression of Livin mRNA and protein were respectively measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Different doses of Livin-siRNA were injected into xenografted tumors in BALB/ c nude mice model in order to confirm the in vivo effect of Livin-siRNA. Results Both Livin mRNA and protein expression were significantly decreased in the experimental group compared with controls (P < 0. 05). In vivo, the tumor size and the signal intensity of luciferase in tumor significantly decreased after Livin-siRNA injection at 50 μmol/ L concentration (P < 0. 05). There were no sig-nificant body weight changes of mice after siRNA injection. Conclusions SiRNA-mediated down-regulation of Livin expression can induce apoptosis in hepatocellular carcinoma in vitro and in vivo, which suggests the im-portant role of Livin gene in hepatocellular carcinoma.
Keywords：Hepatocellular carcinomaMHCC97H cell lineLivinRNA interferenceNude mice
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 351-355 )