The impose of miR-17-5P over cell proliferation and invasion in lung cancer cells A549, SPCA-1 and GLC-82
MENG Lijuan
WANG Jun
FAN Weifei
PU Xiaolin
XU Juqing
WANG Lin
YANG Min
LIU Fuyin
Abstract：Objective The aim of this study was to investigate the effect and molecular mechanism of miR-17-5P over cell proliferation and invasion in lung cancer cells A549 , SPCA-1 and GLC-82. Methods miR-17-5P mimics and inhibitor were synthesized and transfected into lung cancer cells A549, SPCA-1 and GLC-82. The abilities of cell growth,migration and invasion were examined by MTT,Transwell, and Boyden chamber. Westen blot was used to test the expression of cell cycle and EMT-associated proteins. Results Compared with the control group,over expression of miR-17-5P improved A549, SPCA-1 and GLC-82 cells growth and number across polycarbonate membrane(P <0. 001). While miR-17-5P expression was inhibited, cells growth and number across polycarbonate membrane were improved(P<0. 001). Over expression of miR-17-5P upregulate the expression of CCND1,Vimentin and N-CA in A549, SPCA-1 and GLC-82. Meantime, expression of CC-ND1,Vimentin and N-CA was downgraded when expression of miR-17-5P was inhibited. Conclusion miR-17-5P may facilitate cell proliferation, migration and invasion in A549 , SPCA-1 and GLC-82, by which mechanism is related to the improvement of expression of CCNDl,Vimentin and N-CA.
Keywords：miR-17-5Plung cancerproliferationinvasion
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 282-286 )