cfDNA ErbB-2 promoter methylation status in peripheral blood of non-metastatic breast cancer patients ;and its relationship with Her-2 expression in tumor tissue
LIANG Xiao
GUO Meng
SONG Wenzhe
LI Yin
ZHU Zhiqiang
Abstract：Objective To investigate the relationship of methylation of ErbB-2 gene 5 ’ CpG of cfDNA in peripheral blood of non-metastatic breast cancer patients with expression of Her-2 in tumor tissue. Methods I-solate cell-free DNA in peripheral blood of non-metastatic breast cancer patients, analysis methylation of 5’CpG in ErbB-2 gene promoter using bisulfite-sequencing PCR ( BSP) , detect the expression of Her-2 using immuno-histochemistry and FISH. Results Methylation of ErbB-2 gene promoter was only detected in plasma cfDNA of breast cancer patients (19/40), not found in the corresponding peripheral blood cells; Methylation of ErbB-2 gene 5’CpG of cfDNA in peripheral blood of breast cancer patients was negatively correlated with the expression of Her-2 in tumor tissue. Conclusions Methylation of ErbB-2 gene 5’CpG in peripheral blood of breast cancer patients was negatively correlated with the expression of Her-2 in tumor tissue. We can speculate that the methy-lation of ErbB-2 gene 5 ’ CpG in peripheral blood of breast cancer patients can reflect the expression of Her-2 breast cancer tissues to some extent, which may be used as a new method to observe Her-2 status of breast canc-er, and which also can be used to help determine the molecular typing of breast cancer, and used as a new tar-get for breast cancer treatment, and as an early diagnosis indicator of Her-2 positive patients still needs further study.
Keywords：breast cancerBSPHer-2ErbB-2CfDNAmethylation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 212-216 )