Mechanisms of cysteamine inhibit prolactin-dependent tumors
WANG Song
JIANG Zhongjun
BAO Zheng
HU Nie
LUO Ping
Abstract：Objective This study was to investigate the relationship between the effects of CS and prolac-tin-dependent tumor cells in vitro and PRLr,so as to explore inhibition mechanism of CS on prolactin-dependent tumors. Methods Cervical cancer CasKi cells、breast cancer MDA-MB-231 cells and colon cancer SW480 cells were treated with different concentrations of CS,cell proliferation rate was detected by MTT assay,the ex-pression of PRLr was detected by using Western blot,the relationship between cell proliferation rate and expres-sion of PRLr was detected by linear correlation statistical analysis. Results 102 mmol/L CS had highest rate of cell proliferation,but 10 -5 mmol/L and lower had not,it showed a dose-dependent inhibition efficiency;the cell inhibition rates of CS in 24 h to prolactin-dependent tumor cells were the highest,largest on cervical cancer cell, followed on breast cancer,minimum on colon cancer,the same as the expression of PRLr. The cell proliferation inhibition of cervical cancer,breast cancer and the expression of PRLr had a negative correlation. Conclusions The low concentration CS could inhibit prolactin-dependent tumor cells on CasKi cervical cancer,breast cancer MDA-MB-231 cells in vitro,with the relevant of decreased levels intracellular expression of the PRLr.
Keywords：Cysteamineprolactinprolactin receptorcervical cancerbreast cancercolon cancerLinear correlation analysis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 205-208 )