Comparison of growth characteristics of ovarian cancer cells (SK-OV-3)in 2D or 3D culture systems
GONG Zhen
SU Yiping
XU Hanzi
SUN Zhihua
WU Qiang
Abstract：Objective To compare the growth characteristics of ovarian cancer cells in 2D or 3D culture system. Methods Human ovarian cancer cells of SK-OV-3 were maintained under 2D or 3D culture systems. Cells morphology was observed by the inverted microscope and the growth rate was detected by CCK-8 test re-spectively. The cells ’ proliferation and cycle distribution were analyzed by Brdu assay and flow cytometry (FCM)respectively. Results Maintained under 2D culture system,SK-OV-3 cells grew in monolayer;but under 3D culture system,cells formed multi-cellular spheroids (MCS)with a bit slower growth and proliferation rates. The proportion of S/G2-M phase of cells cultured in 2D system was significantly more than those in 3D system [(53. 7 ± 5. 8)% vs. (40. 9 ± 2. 0)%,P<0. 05]. Conclusions The characteristics of ovarian canc-er cells in 2D and 3D culture systems are different. MCS culture system can better mimic the in vivo growth en-vironment of tumors and could be a better model for ovarian cancer research.
Keywords：ovarian cancermulti-cellular spheroidthree-dimensional culture
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 294-297 )