Establishment and primary clinical application of detecting EGFR mutations by high resolution melting analysis
WANG Zhuo
JING Changwen
CAO Haixia
MA Rong
WU Jianzhong
Abstract：Objective To establish a method for detection of EGFR mutations by high resolution melting (HRM)analysis and to evaluate its clinical application value. Methods 200 formalin-fixed paraffin-embedded tissues of NSCLC patients were detected by HRM which have been established. The results of HRM analysis were confirmed by direct DNA sequencing. Results According to the repeatability analysis,it was found that the value of Coefficient of Variance(CV)of Tm and ct was very small. The total EGFR mutation rates were 37. 0% detected by direct sequencing and 40. 0% detected by HRM. The sensitivity of HRM assay was 100% , the specificity of HRM assay was above 95% . Conclusions The method for detection of EGFR mutations by HRM analysis is appropriate for detecting EGFR mutations by HRM analysis. It possesses high specificity,good sensitivity,strong reproducibility,simple operation,saving-time and low cost qualities.
Keywords：EGFRmutationhigh resolution melting
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 236-239 )