Study on associations of expressions of miR-23b-3p and miR-218-5p in cervical squamous cell carcinoma tissues with epithelial-mesenchymal transition,clinicopathological characteristics and prognosis
LIU Shanshan
LIU Dan
SHAN Jinmei
WANG Feng
Abstract：Objective To study the associations of expressions of micro ribonucleic acid-23b-3p(miR-23b-3p)and micro ribonucleic acid-218-5p(miR-218-5p)in cervical squamous cell carcinoma(CSCC)tissues with epithelial-mesenchymal transition(EMT),clinicopathological characteristics and prognosis.Methods A total of 121 patients with CSCC who were admitted to Yancheng Third People's Hospital from June 2016 to June 2019 were selected as the research subjects.During the operation,the CSCC tissues and para-carcinoma tissues(more than 5 cm away from the carcinoma tissues)of these patients were collected,and the expressions of miR-23b-3p,miR-218-5p,epithelial cadherin(E-cadherin)and vimentin were detected by using reverse transcription-quantitative polymerase chain reaction(RT-qPCR).The patients were followed up for 5 years after the operation,and their survival prognosis was recorded.Pearson correlation was used to analyze the correlations between the indicators.Survival curves were plotted by using Kaplan-Meier method,and log-rank test was used for comparison between the groups.Cox regression was used to analyze the factors influencing the prognosis of the CSCC patients.Results The expressions of miR-23b-3p,miR-218-5p and E-cadherin in the CSCC tissues were significantly lower than those in the para-carcinoma tissues(P＜0.05),while the expression of vimentin in the CSCC tissues was significantly higher than that in the para-carcinoma tissues(P＜0.05).In the CSCC tissues,the expressions of miR-23b-3p and miR-218-5p were negatively correlated with the expression of vimentin(P＜0.05),and positively correlated with the level of E-cadherin(P＜0.05).The expressions of miR-23b-3p and miR-218-5p were correlated with International Federation of Gynecology and Obstetrics(FIGO)stage,degree of differentiation,depth of myometrial invasion and lymph node metastasis(P＜0.05),but were not correlated with age and tumor diameter(P＞0.05).The survival prognosis of the patients with high expression of miR-23b-3p(relative expression level ≥1.85,58 cases)was better than that of the patients with low expression of miR-23b-3p(relative expression level＜1.85,61 cases)(log-rank test:x2=7.720,P=0.006).The survival prognosis of the patients with high expression of miR-218-5p(relative expression level ≥1.34,59 cases)was better than that of the patients with low expression of miR-218-5p(relative expression level＜1.34,60 cases)(log-rank test:x2=5.611,P=0.018).The results of multivariate Cox regression analysis showed that lymph node metastasis was an independent risk factor for death in the patients(P＜0.05),and high expressions of E-cadherin,miR-23b-3p and miR-218-5p were protective factors inhibiting death in the patients(P＜0.05).Conclusion Expressions of miR-23b-3p and miR-218-5p are down-regulated in CSCC tissues,and low expressions of miR-23b-3p and miR-218-5p are associated with poor differentiation,advanced FIGO stage,increased depth of myometrial invasion,lymph node metastasis and poor prognosis in CSCC.
Keywords：Cervical squamous cell carcinoma(CSCC)Epithelial-mesenchymal transition(EMT)Clinicopathological characteristicsPrognosisMicro ribonucleic acid-23b-3p(miR-23b-3p)Micro ribonucleic acid-218-5p(miR-218-5p)
Publication Date：2026-02-28
Online Publishing Date：2026-03-31(First online date of this platform, not the publication date of the document)
Pages：6( 196-201 )