Analysis on application value of VITEK®2 AST-YS08 card in drug sensitivity test of non-Candida albicans
LIU Weimin
XU Heping
ZHENG Yanqing
MA Xiaobo
Abstract：Objective To analyze the application value of VITEK®2 AST-YS08 card in drug sensitivity test of non-Candida albicans(NAC).Methods A total of 159 strains of NAC isolated from the normal germfree sites of the inpatients in Xiamen Hospital of Traditional Chinese Medicine and the First Affiliated Hospital of Xiamen University from January 2017 to December 2021 were selected,including 93 strains of C.parapsilosis complex(80 strains of C.parapsilosis,8 strains of C.orthopsilosis,4 strains of C.metapsilosis and 1 strain of Lodderomyces elongisporus),28 strains of C.tropicalis,18 strains of C.glabrata(including 1 strain of C.nivariensis),18 strains of Meyerozyma guilliermondii(including 1 strain of Kodamaea ohmeri)and 2 strains of Pichia kudriavzevii.The minimal inhibitory concentration(MIC)of NAC was tested in parallel using Sensititre YeastOne method(SYO method)and VITEK®2 AST-YS08 card,respectively,and the results were interpreted according to the CLSI-M27S44 standard issued by the Clinical and Laboratory Standards Institute(CLSI).Taking the results of SYO method as the reference,the essential agreement(EA),category agreement(CA),very major error(VME),major error(ME)and minor error(mE)of the results were analyzed between VITEK®2 AST-YS08 card and SYO method.Results Taking the results of SYO method as the reference,the EAs of the results in testing the sensitivity of NAC strains to micafungin,fluconazole,voriconazole,5-flucytosine and caspofungin between VITEK®2 AST-YS08 card and SYO method were 91.82%to 100.00%,and the CAs of the results were 86.79%to 95.12%.Among them,the EA of the results in 5-flucytosine sensitivity test was the highest(100.00%),followed by that in voriconazole sensitivity test(99.29%).The VME of the results in caspofungin sensitivity test by using VITEK®2 AST-YS08 card was associated with the MIC values of the strains of C.orthopsilosis closing to the antimicrobial breakpoints for interpretation.Conclusion VITEK®2 AST-YS08 card is easy to operate and convenient for automation.Taking the results of SYO method as the reference,the CAs of the results in testing the sensitivity of NAC strains to 5-flucytosine,micafungin,fluconazole,caspofungin and voriconazole between VITEK®2 AST-YS08 card and SYO method are higher,and VITEK®2 AST-YS08 card has better clinical application value.
Keywords：Non-Candida albicans(NAC)VITEK®2 AST-YS08 cardDrug sensitivity testEssential agreement(EA)Category agreement(CA)
Publication Date：2025-12-30
Online Publishing Date：2026-01-16(First online date of this platform, not the publication date of the document)
Pages：6( 1385-1390 )