Effects of HSA,IVIG and ATG infusions on blood transfusion compatibility testing
TAI Shengfei
CHENG Tingting
XU Binglin
FU Lihui
MA Chunya
YU Yang
Abstract：Objective To analyze the effects of intravenous infusions of human serum albumin(HSA),intravenous immunoglobulin(IVIG),and rabbit anti-human thymocyte immunoglobulin(ATG)on blood transfusion compatibility testing.Methods The clinical data of 34 patients whose blood samples were received by Department of Transfusion Medicine,the First Medical Center,Chinese People's Liberation Army General Hospital from January 2022 to December 2023 due to the infusions of albumin products or immunoglobulin(Ig)products resulting in incompatible cross-matching,positive results of unexpected antibody screening,and ABO blood group discrepancies were retrospectively analyzed.ABO/RhD typing test,unexpected antibody screening and identification,cross-matching test,direct antiglobulin test(DAT)and acid elution test were performed by using microcolumn gel method.Results Among the 10 patients infused with HSA,IgG-type anti-A was detected in the red blood cell(RBC)eluate of 5 patients with type A and 2 patients with type AB,IgG-type anti-B in the RBC eluate of 1 patient with type B,and agglutination phenomenon of B cells in reverse typing occurred in 1 patient with type B,and both IgG-type anti-A and IgG-type anti-B in the RBC eluate of 1 patient with type AB.Among the 18 patients infused with IVIG,IgG-type anti-A was detected in the RBC eluate of 8 patients with type A,IgG-type anti-A in the RBC eluate of 5 patients with type AB,and both IgG-type anti-A and IgG-type anti-B in the RBC eluate of 1 patient with type AB,and agglutination phenomenon of A1 cells in reverse typing occurred in 3 patients with type A and 1 patient with type AB.Non-specific antibodies were detected in 6 patients infused with ATG.Conclusion After infusions of HSA,IVIG or ATG,the patients are prone to develop positive DAT results,positive results of unexpected antibody screening,ABO blood group discrepancies,and incompatible cross-matching,which interfere with ABO blood typing and cross-matching.
Keywords：Human serum albumin(HSA)Intravenous immunoglobulin(IVIG)Rabbit anti-human thymocyte immunoglobulin(ATG)Transfusion safety
Publication Date：2025-12-30
Online Publishing Date：2026-01-16(First online date of this platform, not the publication date of the document)
Pages：6( 1372-1377 )