Study on correlations of serum miR-27b-3p and miR-142-5p levels with disease progression of clear cell renal cell carcinoma and poor prognosis after retroperitoneal laparoscopic partial nephrectomy

ZHANG Yuan; YAN Yuying; BIAN Yajin; WANG Xueying

doi:10.3969/j.issn.1674-3806.2025.12.08

Abstract：Objective To explore correlations of serum microRNA-27b-3p(miR-27b-3p)and microRNA-142-5p(miR-142-5p)levels with disease progression of clear cell renal cell carcinoma(ccRCC)and poor prognosis after retroperitoneal laparoscopic partial nephrectomy(RLPN).Methods A total of 110 patients with ccRCC who underwent RLPN in the Second Hospital of Qinhuangdao from January 2020 to February 2022 were recruited as ccRCC group,and 55 healthy individuals who underwent physical examination during the same period were selected as control group.The levels of serum miR-27b-3p and miR-142-5p in the ccRCC group before the operation and those in the control group during physical examination were detected by using real-time fluorescence quantitative polymerase chain reaction.The correlations of serum miR-27b-3p and miR-142-5p levels with the clinicopathological indicators of ccRCC were analyzed.Cox regression was used to analyze the factors influencing poor prognosis of the ccRCC patients after RLPN.The receiver operating characteristic(ROC)curve was used to analyze the efficacy of serum miR-27b-3p and miR-142-5p levels in predicting poor prognosis of the ccRCC patients after RLPN.The progression-free survival(PFS)curves of the ccRCC patients with different levels of serum miR-27b-3p and miR-142-5p after RLPN were plotted by K-M method and were compared by using log-rank test.Results Compared with the control group,the ccRCC group had lower serum level of miR-27b-3p,but higher serum level of miR-142-5p,with statistically significant differences between the two groups(P＜0.05).The levels of serum miR-27b-3p and miR-142-5p were significantly correlated with TNM staging and Fuhrman grading(P＜0.05),but were not significantly correlated with gender,age,and tumor site(P＞0.05).The poor prognosis rate of the ccRCC patients after RLPN was 23.64%(26/110).The results of multivariate Cox regression analysis showed that TNM stage T2,Fuhrman gradesⅢ-Ⅳ,and higher serum miR-142-5p level were independent risk factors for poor prognosis in the ccRCC patients after RLPN(P＜0.05),while higher serum miR-27b-3p level was an independent protective factor against poor prognosis in the ccRCC patients after RLPN(P＜0.05).The results of ROC curve analysis showed that the efficacy of the combined use of serum miR-27b-3p and miR-142-5p in predicting poor prognosis of the ccRCC patients after RLPN was better than that of a single indicator(Zcombination of the two indicators-miR-27b-3p=2.148,P=0.032;Zcombination of the two indicators-miR-142-5p=2.329,P=0.020),with a sensitivity of 0.885 and a specificity of 0.857.The progression-free survival prognosis of the high miR-27b-3p group(≥1.37,53 cases)was significantly better than that of the low miR-27b-3p group(＜1.37,57 cases)(P＜0.05).The progression-free survival prognosis of the low miR-142-5p group(＜1.08,58 cases)was significantly better than that of the high miR-142-5p group(≥1.08,52 cases)(P＜0.05).Conclusion The levels of serum miR-27b-3p and miR-142-5p in ccRCC patients are related to the disease progression and prognosis after RLPN.The combined detection of the two indicators is helpful for the early identification of high-risk patients with poor prognosis after RLPN.