Study on mechanisms of lncRNA SNHG16 promoting breast cancer radioresistance by binding to STAU1 to reduce the stability of PRKN mRNA
ZHONG Rui
CHEN Ru
Abstract：Objective To study the mechanisms of long non-coding ribonucleic acid(RNA)(lncRNA)small nucleolar RNA host gene 16(SNHG16)promoting breast cancer radioresistance by binding to host cellular RNA binding protein Staufen 1(STAU1)to reduce the stability of Parkin RBR E3 ubiquitin protein ligase(PRKN)mRNA.Methods The expressions of lncRNA SNHG16 in breast cancer tissues were analyzed by using The Cancer Genome Atlas(TCGA)database and real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expressions of lncRNA SNHG16 in breast cancer cells.MDA-MB-231 and MCF-7 cells with high expression of lncRNA SNHG16 were selected for transfection and were divided into si-NC group(control group)and si-SNHG16 group(experimental group).Cells in logarithmic growth phase were taken for experiment 48 hours after transfection.Cell proliferation was detected by using Cell Counting Kit-8(CCK-8)assay.Cell apoptosis was detected by using flow cytometry.Cell invasion was detected by using Transwell assay.Cell radiosensitivity was detected by using CCK-8 assay.Cellular glucose uptake was determined by using the glucose uptake colorimetric assay kit.The combination of RNA immunoprecipitation(RIP)detection with RNA stability experiment was used to verify the regulatory relationship among lncRNA SNHG16,STAU1 and PRKN.Results lncRNA SNHG16 was highly expressed in breast cancer tissues and cells.Knockdown of lncRNA SNHG16 expression reversed the proliferation,apoptosis,invasion and radioresistance of the breast cancer cells,and inhibited the reprogramming of glucose metabolism in breast cancer cells.The RIP experiment confirmed that there was an interaction between STAU1 and PRKN mRNA,whereas knockdown of lncRNA SNHG16 attenuated this interaction relationship,and knockdown of STAU1 expression also enhanced the stability of PRKN mRNA.Conclusion The highly expressing lncRNA SNHG16 in breast cancer cells binding to STAU1 reduces the stability of PRKN mRNA via a post-transcriptional modification pathway,thus affecting the reprogramming of glucose metabolism and promoting radioresistance in breast cancer.
Keywords：Breast cancer radioresistanceGlycometabolismLong non-coding ribonucleic acid small nucleolar ribonucleic acid host gene 16(lncRNA SNHG16)Host cellular RNA binding protein Staufen 1(STAU1)Parkin RBR E3 ubiquitin protein ligase(PRKN)
Publication Date：2025-10-30
Online Publishing Date：2025-11-13(First online date of this platform, not the publication date of the document)
Pages：7( 1131-1137 )