Study on levels of serum miR-128-3p and miR-211-5p in epilepsy patients and their correlations with the degree of electroencephalogram abnormalities

YU Haiyan; KONG Hui; WANG Rui

doi:10.3969/j.issn.1674-3806.2025.09.14

Abstract：Objective To explore the levels of serum microRNA-128-3p(miR-128-3p)and microRNA-211-5p(miR-211-5p)in epilepsy patients and to analyze their correlations with the the degree of electroencephalogram(EEG)abnormalities.Methods A total of 120 epilepsy patients admitted to Qingdao Chengyang People's Hospital from March 2022 to June 2023 were recruited as epilepsy group,and other 120 healthy individuals who underwent physical examination during the same period were selected as control group.According to the EEG examination results of these epilepsy patients,they were further divided into mild abnormality group(41 patients),moderate abnormality group(46 patients),and severe abnormality group(33 patients).The levels of serum miR-128-3p and miR-211-5p were determined by using quantitative real-time fluorescence polymerase chain reaction(qRT-PCR).The correlations of serum miR-128-3p and miR-211-5p levels with the degree of EEG abnormalities were analyzed by using Spearman rank correlation.The receiver operating characteristic(ROC)curve was used to analyze the efficacy of serum miR-128-3p and miR-211-5p levels in diagnosing severe EEG abnormality.Results The levels of serum miR-128-3p and miR-211-5p in the epilepsy group were significantly lower than those in the control group(P＜0.05).The levels of serum miR-128-3p and miR-211-5p in the epilepsy patients of the severe abnormality group and the moderate abnormality group were significantly lower than those in the epilepsy patients of the mild abnormality group(P＜0.05),and the levels of miR-128-3p and miR-211-5p in the severe abnormality group were lower than those in the moderate abnormality group,with statistically significant differences(P＜0.05).The results of Spearman rank correlation analysis showed that the levels of serum miR-128-3p and miR-211-5p were negatively correlated with the degree of EEG abnormalities(rs=-0.484,P＜0.001;rs=-0.579,P＜0.001).The results of ROC curve analysis showed that the levels of serum miR-128-3p and miR-211-5p could effectively diagnose severe EEG abnormality(P＜0.05),and the diagnostic efficacy of the combination of the two indicators was higher than that of a single indicator(Zcombination of the two indicators-miR-128-3p=2.850,P=0.004;Zcombination of the two indicators-miR-211-5p=2.562,P=0.010),with a sensitivity of 81.82％and a specificity of 79.31％.Conclusion The levels of miR-128-3p and miR-211-5p in the serum of epilepsy patients are decreased,which are negatively correlated with the degree of EEG abnormalities.The combination of the two indicators can better diagnose severe EEG abnormality in the patients.