Association of expressions of KIF18B and ASF1B in endometrial carcinoma tissues with clinicopathological characteristics and survival prognosis in endometrial carcinoma patients

YE Xiaolin; LIANG Xinli; ZHANG Chao

doi:10.3969/j.issn.1674-3806.2025.09.13

Abstract：Objective To explore the expressions of kinesin family member 18B(KIF18B)and anti-silencing function 1B(ASF1B)in endometrial carcinoma(EC)tissues,and to analyze the association of the expressions of KIF18B and ASF1B in endometrial carcinoma tissues with clinicopathological characteristics and survival prognosis.Methods The expression levels of KIF18B,ASF1B mRNA and protein in normal tissues and uterine corpus endometrial carcinoma(UCEC)tissues were analyzed by using bioinformatics methods.The clinical data of 226 patients with EC who were admitted to Xi'an People's Hospital(Xi'an Fourth Hospital)from July 2016 to July 2021 were collected.During the operation,the EC tissues and their adjacent tissues(3 to 5 cm away from the edge of the EC tissues)of the EC patients were collected.The expression levels of KIF18B mRNA and ASF1B mRNA in the EC tissues and their adjacent tissues were detected by using real-time fluorescent quantitative polymerase chain reaction(FQ-PCR).The expressions of KIF18B and ASF1B proteins in the EC tissues and their adjacent tissues were detected by using immunohistochemical staining(SP method).After the operation,the patients were followed up for 3 years and their survival prognosis was recorded.Results The Cancer Genome Atlas(TCGA)database showed that the levels of KIF18B mRNA and ASF1B mRNA in the UCEC tissues were significantly higher than those in the normal tissues(P＜0.05).Clinical Proteomic Tumor Analysis Consortium(CPTAC)database showed that the expression level of ASF1B protein in the UCEC tissues was significantly higher than that in the normal tissues(P＜0.05).The levels of KIF18B mRNA and ASF1B mRNA in the EC tissues were significantly higher than those in their adjacent tissues(P＜0.05).The positive expression rates of KIF18B protein and ASF1B protein in the EC tissues were significantly higher than those in their adjacent tissues(P＜0.05).The expressions of KIF18B and ASF1B proteins were associated with the depth of myometrial infiltration,International Federation of Gynecology and Obstetrics(FIGO)stage and lymph node metastasis(P＜0.05).The results of multivariate Cox regression analysis showed that FIGO stage Ⅲ,positive expression of KIF18B protein,and positive expression of ASF1B protein were independent risk factors for death in the EC patients(P＜0.05).The survival prognosis of the patients with negative expressions of KIF18B and ASF 1B proteins was significantly better than that of the patients with positive expressions of KIF18B and ASF1B proteins(log-rank test:x2=8.518,P=0.004;log-rank test:x2=9.993,P=0.002).Conclusion In EC tissues,both KIF18B and ASF1B are highly expressed,and both are closely associated with the depth of myometrial infiltration,FIGO stage,lymph node metastasis and survival prognosis.