Expressions of serum 8-OHdG and SDF-1 in pediatric patients with neonatal respiratory distress syndrome and their clinical significance

YANG Lijun; SUN Xu'e; CHEN Tingting

doi:10.3969/j.issn.1674-3806.2025.07.14

Abstract：Objective To explore the expressions of serum 8-hydroxy-2'-deoxyguanosine(8-OHdG)and stromal cell-derived factor-1(SDF-1)in pediatric patients with neonatal respiratory distress syndrome(NRDS)and their clinical significance.Methods A total of 108 pediatric patients with NRDS(NRDS group)and 52 normal neonates(control group)who were admitted to Shangluo Central Hospital from July 2021 to May 2024 were recruited.The pediatric patients with NRDS were divided into mild NRDS group(22 patients),moderate NRDS group(50 patients)and severe NRDS group(36 patients)according to their disease severity.Serum 8-OHdG and SDF-1 levels were detected by using enzyme-linked immunosorbent assay.Cox regression was used to analyze the influencing factors of prognosis in the pediatric patients with NRDS.The efficacy of 8-OHdG and SDF-1 levels in predicting death of the pediatric patients with NRDS was analyzed by using receiver operating characteristic(ROC)curve.Results The level of 8-OHdG in the NRDS group was higher than that in the control group[(13.81±3.90)ng/mL vs(3.70±1.47)ng/mL],and the level of SDF-1 in the NRDS group was higher than that in the control group[(12.46±1.62)ng/mL vs(8.23±1.32)ng/mL],with statistically significant differences between the two groups(P＜0.05).The levels of 8-OHdG and SDF-1 in the mild NRDS group,the moderate NRDS group and the severe NRDS group were gradually increased(P＜0.05).The mortality rate of the 108 pediatric patients with NRDS within 28 days after admission was 25.00％(27/108).The results of multivariate Cox regression analysis showed that severe NRDS,and higher 8-OHdG and SDF-1 levels were independent risk factors for death in the pediatric patients with NRDS(P＜0.05).The results of ROC curve analysis showed that the levels of 8-OHdG and SDF-1 were helpful for predicting death of the pediatric patients with NRDS(P＜0.05).The predictive efficacy of the combination of the two indicators[AUC(95％CI)=0.925(0.859-0.967)]was higher than that of a single indicator(P＜0.05),with a sensitivity of 92.59％and a specificity of 77.78％.Conclusion The elevated serum 8-OHdG and SDF-1 levels are closely related to the disease severity and the increase of death risk in pediatric patients with NRDS.The combination of the two indicators is more effective than a single indicator in predicting prognosis of the pediatric patients with NRDS.