A study on application value of mass spectrometry nucleic acid detection technology in rapid identification of carbapenemase genotypes in Klebsiella pneumoniae
YE Lin
LIANG Xinyi
DENG Xinyi
ZHAO Shulong
SONG Shuang
SUN Jingfang
KANG Haiquan
Abstract：Objective To establish a method for rapid identification of carbapenemase genotypes in Klebsiella pneumoniae on the basis of mass spectrometry nucleic acid detection technology.Methods A total of 56 non-redundant strains of carbapenem-resistant Klebsiella pneumoniae(CRKP)isolated in the Affiliated Hospital of Xuzhou Medical University from January 2021 to September 2023 were collected.Bacterial identification and antimicrobial susceptibility analysis were performed using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry(MALDI-TOF MS)and VITEK-2 Compact system.Specific single-base extension primers were designed,and a multiplex polymerase chain reaction(PCR)combined with mass spectrometry nucleic acid detection technology was employed to detect the characteristic peaks of carbapenemase genotypes in a single reaction system.The consistency of the results of identifying the carbapenemase genotypes in CRKP by using mass spectrometry nucleic acid detection technology and using PCR was analyzed.Results Among the 56 strains of CRKP,there were 14 strains with KPC,14 strains with NDM,14 strains with OXA-232,and 14 strains with co-production of KPC+NDM.There was no cross-interference among the characteristic peaks of each subtype.The consistency between the detection results using mass spectrometry nucleic acid detection technology and using PCR for KPC-,OXA-232-and(KPC+NDM)-resistant strains was 100.00％.Only 1 strain with NDM was mistakenly classified as NDM+OXA co-production strain,and the overall detection consistency rate was 98.21％(55/56).The results of the drug sensitivity test showed that the resistance rate of the strains with KPC or the strains with NDM to imipenem was 100.00％,while the resistance rate of the strains with OXA-232 to imipenem was 0.Conclusion The mass spectrometry nucleic acid detection technology can efficiently and accurately identify the carbapenemase genotypes in CRKP,which is highly consistent with the results of PCR.This mass spectrometry nucleic acid detection technology provides a reliable tool for rapid clinical typing and drug resistance monitoring.
Keywords：Klebsiella pneumoniaeCarbapenemaseMatrix-assisted laser desorption/ionization-time-of-flight mass spectrometry(MALDI-TOF MS)Mass spectrometry nucleic acid detection technology
Publication Date：2025-07-30
Online Publishing Date：2025-08-27(First online date of this platform, not the publication date of the document)
Pages：7( 729-735 )