Analysis on expressions of PIM2 and PFKFB3 in tissues of patients with non-small cell lung cancer and association of these expressions with clinicopathological features and survival prognosis
WEI Jiali
HUANG Qinrong
YANG Jinping
HUANG Na
YANG Li
Abstract：Objective To analyze the expressions of proviral integration site for Moloney murine leukemia virus 2(PIM2)and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3(PFKFB3)in tissues of patients with non-small cell lung cancer(NSCLC)and the association of these expressions with clinicopathological features and survival prognosis.Methods A total of 98 patients with NSCLC who were admitted to the First People's Hospital of Guangyuan from January 2020 to March 2021 were selected.The clinical data of the patients were collected.During the operation,NSCLC tissues and pericarcinomatous tissues(＞3 cm away from the cancer tissues)were collected.The expressions of PIM2 and PFKFB3 were detected by immunohistochemical staining.The patients were followed up for 3 years after the operation.The survival status of the patients was recorded.Cox regression was used to analyze the factors influencing the survival prognosis of the NSCLC patients.The survival curve was plotted using Kaplan-Meier method,and log-rank test was used for inter-group comparison.Results The positive expression rates of PIM2 and PFKFB3 in NSCLC tissues were significantly higher than those in pericarcinomatous tissues(71.43％vs 14.29％,68.73％vs 16.33％;P＜0.05).The expressions of PIM2 and PFKFB3 were related to clinical stage,lymph node metastasis and degree of differentiation(P＜0.05),but were not associated with age,gender,tumor diameter and pathological type(P＞0.05).The results of multivariate Cox regression analysis showed that clinical stages Ⅲ and Ⅳ,lymph node metastasis,positive expression of PIM2 and positive expression of PFKFB3 were independent risk factors for death in the NSCLC patients(P＜0.05).The 3-year overall survival rate of the 98 NSCLC patients was 54.08％.The survival prognosis of the patients with negative expressions of PIM2 and PFKFB3 was better than that of the patients with positive expressions of PIM2 and PFKFB3(log-rank test:x2=5.777,P=0.016;x2=5.422,P=0.020).Conclusion Positive expressions of PIM2 and PFKFB3 are associated with the adverse clinicopathological features and poor prognosis of the patients.
Keywords：Proviral integration site for Moloney murine leukemia virus 2(PIM2)6-phosphofructo-2-kinase/fructose-26-bisphosphatase 3(PFKFB3)Non-small cell lung cancer(NSCLC)Clinicopathological features
Publication Date：2025-06-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 662-666 )